The Science Seminar Series: September 17, 2009
Biological Role and Detection of Nitric Oxide and Activity of
Dr. Yakov Woldman
Department of Chemistry
Time: 4:00 -5:00pm
In this presentation author discusses the role of nitric oxide in various physiological processes and methods of its detection. The challenge in the detection of nitric oxide originates form extremely low (sub-nanomolar)physiological concentrations. The method suggested allows for measuring nitric oxide production using its natural target - soluble guanylyl cyclase (sGC). Guanylyl cyclase catalyzes the conversion of guanosine triphopshate into guanosine 3', 5'-cyclic monophosphate and inorganic pyrophosphate. The rate of the reaction increases about 200 times upon NO binding with sGC, providing thereby sensor for nitric oxide. The luminescent detection of the above reaction was accomplished by converting inorganic pyrophosphate into ATP with the help of enzyme ATP sulfurylase; ATP, in its turn, was detected in chemiluminescent luciferin-luciferase reaction. The developed luminescent approach allows for measuring the rates of NO generation as low as 1 nM/min.