The Science Seminar Series: March 26, 2009 4pm
Reading Chemical "Fine Print": The Key to Exploiting Nature's Isotopic Complexity
Dr. Alan G. Marshall
Ion Cyclotron Resonance Program, National High Magnetic Field Lab, Tallahassee, FL
Department of Chemistry and Biochemistry, Florida State University, Tallahassee, FL
Time: 4:00 - 5:00pm
Most mass analysis relies on "nominal" mass accuracy (i.e., to within 1 Da). However, more and more applications are based on much more accurate mass measurement. Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS) offers 10-100 times higher mass resolving power than other mass analyzers.
High mass resolving power (m/?m50% > 400,000 over a wide mass range) offers two major advantages. First, it becomes possible to separate complex mixtures without prior chromatographic or gel separation. Second, elemental composition may be determined from accurate (to <1 ppm) mass measurement alone for unknown molecules up to ~1,000 Da. Examples from environmental, petrochemical, analytical, and biological (especially proteomics) problems will be presented, including world records for mass resolution.
Supported by NSF (DMR-06-54118), NIH (GM-78359), Florida State U., and the Nat'l High Magnetic Field Lab
Marshall, A. G.; Hendrickson, C. L.; Jackson, G. S. "Fourier Transform Ion Cyclotron Resonance Mass Spectrometry: A Primer," Mass Spectrom. Rev. 1998, 17, 1-35.
Marshall, A. G. "Milestones in FT-ICR MS Technique Development," Int. J. Mass Spectrom. 2000, 200, 331-356.
Marshall, A. G.; Rodgers, R. P. "Petroleomics: The Next Grand Challenge for Chemical Analysis," Acc. Chem. Res. 2004, 37, 53-59.
Cooper, H. J.; Håkansson, K.; Marshall, A. G. "The Role of Electron Capture Dissociation in Biomolecular Analysis," Mass Spectrom. Rev. 2005, 24, 201-222.
Oomens, J.; Polfer, N.; Moore, D. T.; Marshall, A. G.; Eyler, J. R.; Meijer, G.; von Helden, G. "Charge-State Resolved Mid-Infrared Spectroscopy of a Gas-Phase Protein," Phys. Chem. Chem. Phys. 2005, 7, 1345-1348
Purcell, J. M.; Hendrickson, C. L.; Rodgers, R. P.; Marshall, A. G. "Atmospheric Pressure Photoionization FT-ICR Mass Spectrometry for Complex Mixture Analysis," Anal. Chem. 2006, 78, 5906-5912.
Tsybin, Y. O.; He, H.; Emmett, M. R.; Hendrickson, C. L.; Marshall, A. G. "Ion Activation in Electron Capture Dissociation To Distinguish between N-Terminal and C-terminal Product Ions," Anal.Chem. 2007, 79, 7596-7602.
He, H.; Conrad, C. A.; Nilsson, C. L.; Ji, Y.; Schaub, T. M.; Marshall, A. G.; Emmett, M. R., "Method for Lipidomic Analysis: p53 Expression Modulates Sulfatide, Ganglioside and Phospholipid Composition of U87 MG Glioblastoma Cells," Anal.Chem. 2007, 79, 8423-8430.
Schaub, T. M.; Hendrickson, C. L.; Horning, S.; Quinn, J. P.; Senko, M. W.; Marshall, A. G. "High Performance Mass Spectrometry: Fourier Transform Ion Cyclotron Resonance at 14.5 Tesla," Anal. Chem. 2008, 80, 3985-3990.